Methylen Blue Usage In Evaluation Of Traumatic Necrosis In Rabbit Liver Injury

BACKGROUND
In this study, the efficacy of methylene blue in the differentiation of devascularized and vital areas in a traumatic liver model in rabbits was investigated.
METHODS
Twenty healthy rabbits were selected and randomly divided into two equal groups. Surgery was performed through an upper abdominal mid-line incision 5 cm in length. After induction of liver injury, diluted methylene blue was injected through the portal vein and staining onset and discoloration times were recorded.
RESULTS
All 20 rabbits survived until the end of the experiment. In cases, non-perfused areas of the liver remained unstained. In control rabbits, liver parenchyma was stained homogeneously. The mean onset time of staining in normal parenchyma was 18.1±1.3 sec in cases and 17.7±1.4 sec in controls (p=NS). The mean discoloration time was 53.1±3.7 sec in cases and 53.1±3.2 sec in controls (p=NS).
CONCLUSION
In major liver injuries requiring surgical intervention, methylene blue injection can be used for detection of non-perfused areas in the liver.

Tavþan Modeli Üzerinde Deneysel Olarak Gerçekleþtirilen Karaciðer Hasarýndaki Travmatik Nekrozun Deðerlendirilmesinde Metilen Mavisi Kullanýmý

AMAÇ
Bu çalýþmada, metilen mavisinin travmatik tavþan karaciðerinin vital ve devaskülarize alanlarýnýn ayýrýmý konusundaki etkisi araþtýrýldý.
GEREÇ VE YÖNTEM
Yirmi adet saðlýklý tavþan rastgele iki eþit gruba ayrýldý. Cerrahi iþlem, karýn üst bölgede orta hattan gerçekleþtirilen 5 cm uzunluðunda bir insizyonla uygulandý. Karaciðer hasarý oluþturulduktan sonra, portal ven yoluyla dilüe edilmiþ metilen mavisi enjekte edildi ve boyanmaya baþlanma zamanlarý ile boyanýn kaybolduðu zamanlar kaydedildi.
BULGULAR
Yirmi tavþanýn hepsi de deney bitimine kadar yaþadý. Deneklerde karaciðerin perfüze olmayan alanlarý boyanmadý. Kontrol tavþanlarýnda, karaciðer parankimi homojen olarak boyandý. Normal parankimdeki ortalama boyanmaya baþlama zamaný, deneklerde 18,1±1,3 sn olurken kontrollerde de 17,7±1,4 sn oldu (p=AD). Ortalama diskolorasyon zamaný, deneklerde 53,1±3,7 sn ve kontrollerde de 53,1±3,2 sn oldu (p=AD).
SONUÇ
Cerrahi giriþim gerektiren majör karaciðer yaralanmalarýnda, karaciðerin perfüze olmayan alanlarýnýn saptanmasý için metilen mavisi enjeksiyonu kullanýlabilir.