Ulus Travma Acil Cerrahi Derg. 2020; 26(3): 351-360 | DOI: 10.14744/tjtes.2020.81456

Sýçan arka uzuv kas modelinde iskemi/reperfüzyon hasarýna yönelik likopenin koruyucu etkisi

Mehmet Kiriþçi¹, Bülent Güneri², Muhammed Seyithanoðlu³, Ülkü Kazancý⁴, Adem Doðaner⁵, Hakan Güneþ^6
1Kahramanmaraþ Sütçü Ýmam Üniversitesi Týp Fakültesi, Kalp Damar Cerrahi Anabilim Dalý, Kahramanmaraþ
²Kahramanmaraþ Sütçü Ýmam Üniversitesi Týp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalý, Kahramanmaraþ
³Kahramanmaraþ Sütçü Ýmam Üniversitesi Týp Fakültesi, Týbbi Biyokimya Anabilim Dalý, Kahramanmaraþ
⁴Kahramanmaraþ Sütçü Ýmam Üniversitesi Týp Fakültesi, Týbbi Patoloji Anabilim Dalý, Kahramanmaraþ
⁵Kahramanmaraþ Sütçü Ýmam Üniversitesi Týp Fakültesi, Biyoistatistik ve Týbbi Biliþim Anabilim Dalý, Kahramanmaraþ
⁶Kahramanmaraþ Sütçü Ýmam Üniversitesi Týp Fakültesi, Kardiyoloji Anabilim Dalý, Kahramanmaraþ

AMAÇ: Önemli bir mortalite ve morbidite sebebi olan iskelet kasýnýn akut iskemi/reperfüzyon (I/R) hasarý oksidatif stres ile iliþkilidir. Etkin bir antioksidatif ve bir tür karotenoid pigment olan likopen meyve ve sebzelerde bulunmaktadýr. Çalýþmamýz sýçan arka uzuv kas modelinde likopenin Ý/R hasarýna karþý koruyucu etkisini incelemektedir.
GEREÇ VE YÖNTEM: Otuz iki adet Wistar-albino türü sýçan kontrol, likopen, Ý/R ve Ý/R+likopen gruplarýna rasgele daðýtýldý. Likopen ve Ý/R+likopen gruplarýndaki sýçanlar deneyden önceki 15 gün boyunca aðýzdan günde, 10 mg/kg miktarýnda likopen aldý. Tüm sýçanlara genel anestezi altýnda böbrek altý seviyesinde abdominal aorta diseksiyonu uygulandý. I/R ve I/R+likopen gruplarýnda aorta, böbrek altý seviyesinde iki saat süre ile klemp kullanýlarak kapatýldý. Ardýndan bu gruplarda iki saat süre ile reperfüzyona olanak saðlandý. Feda edildikten sonra sýçanlarýn arka uzuv kasýndan biyokimyasal ve histopatolojik inceleme için örnekler alýndý.
BULGULAR: I/R+likopen grubunda serum ve doku malondialdehit ve iskemi modifiye albumin düzeyleri I/R grubuna kýyasla anlamlý derecede düþüktü (p<0.001). I/R grubunda serum glutatyon peroksidaz (GSH-Px) düzeylerinin kontrol ve I/R+likopen gruplarýndakine göre anlamlý derecede düþük olduðu belirlendi (p<0.05). I/R grubunda doku GSH-Px düzeyleri likopen grubundakine göre anlamlý derecede düþüktü (p=0.003). Diðer üç gruba oranla I/R grubunda serum süperoksit dismutaz (SOD) düzeylerinin anlamlý derecede düþük olduðu saptandý (p<0.001). I/R grubunda doku SOD seviyeleri kontrol ve likopen gruplarýndakine göre anlamlý derecede düþüktü (p=0.005). Histopatolojik incelemelerde I/R+likopen grubunda I/R hasarýna baðlý enflamatuvar deðiþimlerin belirgin olarak az olduðu görüldü.
TARTIÞMA: Çalýþmamýz sýçan iskelet kasý modelinde likopenin I/R hasarýna karþý hücre koruyucu etkinliðini göstermektedir.

Anahtar Kelimeler: Antioksidanlar, iskemi; likopen; reperfüzyon hasarý; turnike.

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The protective effects of lycopene on ischemia/reperfusion injury in rat hind limb muscle model

Mehmet Kiriþçi¹, Bülent Güneri², Muhammed Seyithanoðlu³, Ülkü Kazancý⁴, Adem Doðaner⁵, Hakan Güneþ^6
1Department of Cardiovascular Surgery, Kahramanmaraþ Sütçü Ýmam University Faculty of Medicine, Kahramanmaraþ-Turkey
²Department of Orthopedics and Traumatology, Kahramanmaraþ Sütçü Ýmam University Faculty of Medicine, Kahramanmaraþ-Turkey
³Department of Medical Biochemistry, Kahramanmaraþ Sütçü Ýmam University Faculty of Medicine, Kahramanmaraþ-Turkey
⁴Department of Medical Pathology, Kahramanmaraþ Sütçü Ýmam University Faculty of Medicine, Kahramanmaraþ-Turkey
⁵Department of Biostatistics and Medical Informatics, Kahramanmaraþ Sütçü Ýmam University Faculty of Medicine, Kahramanmaraþ-Turkey
⁶Department of Cardiology, Kahramanmaraþ Sütçü Ýmam University Faculty of Medicine, Kahramanmaraþ-Turkey

BACKGROUND: Acute ischemia/reperfusion (I/R) injury of skeletal muscle, an important mortality and morbidity cause, is associated with oxidative stress. Lycopene is a carotenoid pigment with potent antioxidant activity and is found in vegetables and fruits. This study aims to investigate the protective effects of lycopene against I/R injury in rat hind limb muscle model.
METHODS: Thirty-two Wistar-albino rats were randomly allocated to control, lycopene, I/R and I/R+lycopene groups. In lycopene and I/R+lycopene groups, the rats received 10 mg/kg/day lycopene orally for 15 days before the experiment. Dissection around abdominal aorta at the infrarenal level was performed in all rats under general anesthesia. The aorta was clamped at the infrarenal level in the I/R and I/R+lycopene groups for two hours. Then, reperfusion was allowed for two hours in these groups. Samples were obtained from the hind limb muscles of rats after sacrifice for biochemical and histopathological analyses.
RESULTS: Serum and tissue malondialdehyde and ischemia-modified albumin levels were significantly lower in the I/R+lycopene group compared to I/R group (p<0.001). Serum glutathione peroxidase (GSH-Px) levels were significantly lower in the I/R group compared to those in control and I/R+lycopene groups (p<0.05). Tissue GSH-Px levels were significantly lower in the I/R group compared to the Lycopene group (p=0.003). Serum superoxide dismutase (SOD) levels were significantly lower in the I/R group compared to three groups (p<0.001). Tissue SOD levels were significantly lower in the I/R group compared to those in control and Lycopene groups (p=0.005). Histopathological assessments revealed that inflammatory changes following I/R injury were significantly reduced in the I/R+lycopene group.
CONCLUSION: The findings obtained in this study show lycopene’s cytoprotective activity against I/R injury in rat skeletal muscle model.

Keywords: Antioxidants, ischemia; lycopene; reperfusion injury; tourniquets.

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