Ulus Travma Acil Cerrahi Derg. 2024; 30(6): 375-381 | DOI: 10.14744/tjtes.2024.27388

Etomidat ratlarda over iskemi-reperfüzyon hasarýný hafifletir

Vildan Kölükçü¹, Mehtap Gürler Balta¹, Hakan Tapar¹, Tuðba Karaman¹, Serkan Karaman¹, Velid Unsal², Fikret Gevrek³, Muzaffer Katar^4
1Tokat Gaziosmanpaþa Üniversitesi Týp Fakültesi, Anestezi ve Reanimasyon Anabilim Dalý, Tokat, Türkiye
²Mardin Artuklu Üniversitesi, Saðlýk Bilimleri Fakültesi ve Merkezi Araþtýrma Laboratuvarý, Mardin, Türkiye
³Tokat Gaziosmanpaþa Üniversitesi Týp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalý, Tokat, Türkiye
⁴Tokat Gaziosmanpaþa Üniversitesi Týp Fakültesi, Biyokimya Anabilim Dalý, Tokat, Türkiye

AMAÇ: Deneysel over iskemi reperfüzyon hasarýnda etomidat’ýn oksidatif hasara koruyucu etkisini araþtýrmayý amaçladýk.
GEREÇ VE YÖNTEM: Toplam 24 diþi rat üç gruba randomize edildi. Grup 1 kontrol grubu olarak atandý. Grup 2'ye over torsiyon/detorsiyon modeli uygulandý. Grup 3'te Grup 2'deki sýçanlara benzer prosedürler uygulandý. Ayrýca Grup 3'te over detorsiyonundan 30 dakika önce 4 mg/kg etomidate intraperitoneal olarak uygulandý. Kan örnekleri alýnarak lipid peroksidayon düzeyi, proinflamatuvar sitokin seviyesi ve antioksidan enzim aktivitesi deðerlendirildi. Ek olarak over dokusundaki hasar histopatolojik olarak skorlandý.
BULGULAR: Kan örneklerinin biyokimyasal analizinde, interlökin-1 beta (IL-1beta), interlökin-6 (IL-6) ve tümör nekroz faktör-alfa (TNF-alfa) dahil olmak üzere proinflamatuar sitokinlerin düzeylerinde Grup 3’de Grup 2’e göre azalma olduðunu gözlemlendi (sýrasýyla 0.005, p: 0.016 ve p<0.001). Benzer þekilde malondialdehit (MDA) düzeyinde de Grup 3’de Grup 2’ye göre azalma olduðu belirlendi (p<0.001). Süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-PX) dahil antioksidan enzim aktivitesinde ise Grup 3’de Grup 2’e göre ciddi düzeyde artýþ izlendi (sýrasýyla p: 0.031 ve p: 0.001). Ayrýca Grup 3; Grup 2 ile karþýlaþtýrýldýðýnda foliküler dejenerasyon, damar týkanýklýðý, kanama ve inflamasyon dahil histopatolojik skorlarda anlamlý bir azalma vardý (sýrasýyla p<0.001, p<0.001, p<0.001 ve p: 0.001).
SONUÇ: Etomidat, rat over torsiyon/detorsiyon modelinde histopatolojik ve biyokimyasal bulgularý iyileþtirerek iskemi reperfüzyon hasarýný ha-fifletmektedir.

Anahtar Kelimeler: Etomidat, iskemi reperfüzyon hasarý; oksidatif stres; over torsiyon ve detorsion.

---

Etomidate alleviates ovarian ischemia-reperfusion injury in rats

Vildan Kölükçü¹, Mehtap Gürler Balta¹, Hakan Tapar¹, Tuðba Karaman¹, Serkan Karaman¹, Velid Unsal², Fikret Gevrek³, Muzaffer Katar^4
1Department of Anesthesiology and Reanimation, Tokat Gaziosmanpaþa University Faculty of Medicine, Tokat-Türkiye
²Mardin Artuklu University, Faculty of Health Sciences and Central Research Laboratory, Mardin-Türkiye
³Department of Histology And Embryology, Tokat Gaziosmanpaþa University Faculty of Medicine, Tokat-Türkiye
⁴Department of Biochemistry, Tokat Gaziosmanpaþa University Faculty of Medicine, Tokat-Türkiye

BACKGROUND: This study investigates the protective effects of etomidate against oxidative damage in an experimental model of ovarian ischemia-reperfusion injury.
METHODS: A total of 24 female rats were randomized into three groups. Group 1 served as the control. Group 2 underwent an ovarian torsion/detorsion procedure. Group 3 underwent similar procedures as Group 2; additionally, 4 mg/kg of etomidate was administered intraperitoneally 30 minutes before ovarian detorsion. Blood samples were analyzed for lipid peroxidation, pro-inflammatory cytokine levels, and antioxidant enzyme activity.Furthermore, histopathological scoring was performed to evaluate tissue damage in the ovaries.
RESULTS: Biochemical analysis of blood samples revealed reductions in pro-inflammatory cytokines, including interleukin-1 Beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), in Group 3 compared to Group 2 (p=0.005, p=0.016, and p<0.001, respectively). Additionally, a decrease in malondialdehyde (MDA) levels was observed in Group 3 compared to Group 2 (p<0.001). In contrast, activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), were significantly increased in Group 3 compared to Group 2 (p=0.031 and p=0.001, respectively). Furthermore, Group 3 demonstrated notable reductions in histopathological scores for follicular degeneration, vascular occlusion, bleeding, and inflammation compared to Group 2 (p<0.001, p<0.001, p<0.001, and p=0.001, respectively).
CONCLUSION: Etomidate alleviates ischemia-reperfusion injury in a rat ovarian torsion-detorsion model by improving both histo-pathological and biochemical outcomes.

Keywords: Etomidate, ovarian torsion and detorsion; ischemia-reperfusion injury; oxidative stress.

---

ATIF KOPYALA