Ulus Travma Acil Cerrahi Derg. 2022; 28(6): 736-742 | DOI: 10.14744/tjtes.2021.01954

Sýçanlarda deneysel travmatik beyin hasarý modelinde adrenomedullin’in nöroprotektif etkileri

Gökçen Emmez¹, Erkut Baha Bulduk², Zuhal Yýldýrým^3
1Gazi Üniversitesi Týp Fakültesi, Anestezi ve Reanimasyon Anabilim Dalý, Ankara
²Atýlým Üniversitesi Týp Fakültesi, Beyin ve Sinir Cerrahisi Anabilim Dalý, Ankara
³Etimesgut Halk Saðlýðý Laboratuvarý, Ankara

AMAÇ: Travmatik beyin hasarý beyne; hücre ölümü, kan-beyin bariyerinin bozulmasý ve iskemi dahil olmak üzere çeþitli þekillerde zarar verir. Bu çalýþmada, sýçan modellerinde kafa travmasý sonrasý adrenomedüllinin (AM) oksidatif stres ve enflamasyon üzerindeki etkilerini araþtýrdýk. GEREÇ VE YÖNTEM: On sekiz erkek yetiþkin Wistar-Albino sýçan rastgele üç gruba (n=6) ayrýldý. Kontrol (C) grubuna herhangi bir travma uygu-lanmadý. Travma grubundaki sýçanlara Marmarau travma modeline uygun travma uygulandý. Adrenomedüllin tedavi grubundaki sýçanlara ise travma grubuna ek olarak travma sonrasý 12 μg/kg ip adrenomedüllin uygulandý. Tüm gruplardaki sýçanlar yedi gün boyunca takip edildi ve sonrasýnda sakrifiye edilip beyin dokularý ve kan örnekleri alýndý.
BULGULAR: Travma grubunda hem doku hem de serum MDA, TNF-α ve IL-6 seviyeleri kontrol grubuna göre anlamlý olarak arttý (p<0.05). AM ile tedavi edilen grupta, serum TNF-α seviyeleri travma grubuna göre anlamlý olarak azaldý (p<0.05). Travma grubunda hem doku hem de serum GSH düzeyleri kontrol grubuna göre anlamlý olarak azaldý (p<0.05). Travma grubunda serum vit D3 seviyeleri kontrol grubuna göre anlamlý olarak azaldý (p<0.05). AM ile tedavi edilen grupta, hem doku hem de serum GSH seviyeleri, travma grubuna göre anlamlý olarak arttý (p<0.05).
TARTIÞMA: Bu sonuçlar AM’nin bir sýçan modelinde travmatik beyin hasarý üzerinde nöroprotektif etkilere sahip olduðunu göstermektedir.

Anahtar Kelimeler: Adrenomedüllin, antioksidan; enflamasyon; oksidatif stres; travmatik beyin hasarý.

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Neuroprotective effects of adrenomedullin in experimental traumatic brain injury model in rats

Gökçen Emmez¹, Erkut Baha Bulduk², Zuhal Yýldýrým^3
1Department of Anesthesiology and Reanimation, Gazi University Faculty of Medicine, Ankara-Turkey
²Department of Neurosurgery, Atýlým University Faculty of Medicine, Ankara-Turkey
³Etimesgut Public Health Laboratory, Ankara-Turkey

BACKGROUND: Traumatic brain injuries cause damages in the brain in several ways, which include cell death because of edema, disruption of the blood–brain barrier, shear stress, and ischemia. In this study, we investigated the effects of adrenomedullin (AM) on oxidative stress and inflammation after head traumas in a rat model.
METHODS: Eighteen male adult Wistar albino rats were randomized into three groups (n=6). No traumas were applied to the con-trol (C) group. Traumas were applied in line with Marmarau trauma model in the trauma group. The rats in the AM treatment group were treated with post-traumatic 12 μg/kg i.p. AM in addition to the trauma group. The rats were followed for 7 days in all groups and were then sacrificed. Brain tissues and blood samples were taken.
RESULTS: In the trauma group, both tissue and serum MDA, TNF-α, and IL-6 levels were significantly increased compared to the control group (p<0.05). In the AM-treated group, serum TNF-α levels were significantly decreased compared to the trauma group (p<0.05). In the trauma group, both tissue and serum GSH levels were significantly decreased compared to the control group (p<0.05). In the trauma group, serum Vitamin D3 levels were significantly decreased compared to the control group (p<0.05). In the AM-treated group, both tissue and serum GSH levels were significantly increased compared to the trauma group (p<0.05).
CONCLUSION: These results indicate that AM has neuroprotective effects on traumatic brain injury in a rat model.

Keywords: Adrenomedullin, inflammation; oxidative stress; traumatic brain injury.

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