Ulus Travma Acil Cerrahi Derg. 2016; 22(5): 423-431 | DOI: 10.5505/tjtes.2016.44575

Deneysel spinal kord travmasý sonrasý resveratrol ve kuersetin’in etkinliðinin araþtýrýlmasý

Ulvi Çiftçi¹, Emre Delen², Murat Vural³, Onur Uysal⁴, Didem Turgut Coþan⁵, Canan Baydemir⁶, Fulya Doðaner^5
1Bakýrköy Dr. Sadi Konuk Eðitim Ve Araþtýrma Hastanesi, Nöroþirürji Servisi, Ýstanbul
²Edirne Devlet Hastanesi, Nöroþirürji Servisi, Edirne
³Eskiþehir Osmangazi Üniversitesi Týp Fakültesi, Nöroþirürji Ana Bilim Dalý, Eskiþehir
⁴Eskiþehir Osmangazi Üniversitesi Saðlýk Hizmetleri Meslek Yüksekokulu, Eskiþehir
⁵Eskiþehir Osmangazi Üniversitesi Týp Fakültesi, Medikal Biyoloji Ana Bilim Dalý, Eskiþehir
⁶Kocaeli Üniversitesi Týp Fakültesi, Biyoistatistik Ana Bilim Dalý, Kocaeli

AMAÇ: Bu çalýþmada, doðal antioksidanlardan olan resveratrol ve kuersetin’in, deneysel spinal kord travmasý oluþturulmuþ sýçanlarda, oksidatif stres ve sekonder doku hasarý üzerine olan etkilerini araþtýrmak amaçlandý.
GEREÇ VE YÖNTEM: Bu deneysel çalýþmada, 42 adet Sprague-Dawley cinsi erkek sýçan kullanýldý. Spinal kord hasarý klip kompresyon metodu kullanýlarak T4-T5 seviyesinden yapýldý. Çalýþma kontrol, travma, travma+çözücü, travma+resveratol, travma+kuersetin ve travma+kombine (resveratrol ve kuersetin) olmak üzere 6 grup üzerinden yürütüldü. Ýþlemden 48 saat sonra tüm sýçanlar sakrafiye edildi. Resveratrol ve kuersetin’in serum ve dokuda total antioksidan kapasite düzeyi ve paraoksonaz enzim aktivitesine ve histopatolojik bulgulara olan etkisi araþtýrýldý.
BULGULAR: Sonuç olarak resveratrol, kuersetin ve kombine tedavi verilen gruplarda TAOK düzeyi ve PON aktivitesi deðerlerinde travma grubuna göre anlamlý bir artma saptandý. Resveratrol ve kuersetin gruplarý arasýnda TAOK düzeyi ve PON aktivitesi açýsýndan anlamlý bir farklýlýk yoktu. Çözücü grubunda TAOK düzeyi ile PON aktivitesi travma grubuna göre anlamlý derecede yüksekti. Histopatolojik incelemede ise sadece çözücü, resveratrol, kuersetin ve kombine tedavi gruplarýnda PMNL infiltrasyonunun azaldýðý görüldü.
TARTIÞMA: Çalýþmamýzdaki biyokimyasal ve histolojik deðerlendirmede resveratrol ve kuersetin uygulamasýnýn spinal kord travmasýnda görülen ikincil hasardan, spinal kordu korumak için kullanýlabileceði sonucuna varýlmýþtýr.

Anahtar Kelimeler: Antioksidan, deneysel spinal kord hasarý, kuersetin, resveratrol.

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Efficiacy of resveratrol and quercetin after experimental spinal cord injury

Ulvi Çiftçi¹, Emre Delen², Murat Vural³, Onur Uysal⁴, Didem Turgut Coþan⁵, Canan Baydemir⁶, Fulya Doðaner^5
1Bakýrköy Dr Sadi Konuk Education And Research Hospital, Departmant Of Neurosurgery, Ýstanbul
²Edirne State Hospital, Departmant Of Neurosurgery, Edirne
³Eskiþehir Osmangazi University, Medical Faculty, Departmant Of Neurosurgery, Eskisehir
⁴Eskiþehir Osmangazi University Vocational School Of Health Services, Eskisehir
⁵Eskiþehir Osmangazi University Medical Faculty Department Of Medical Biology, Eskiþehir
⁶Kocaeli University Medical Faculty, Department Of Biostatistics, Kocaeli

BACKGROUND: The aim of this study was to investigate the effect of natural antioxidants resveratrol and quercetin on oxidative stress and secondary cell damage in rats with acute spinal cord injury.
METHODS: In this experimental study, 42 male Sprague-Dawley rats were used. Spinal cord injury was performed with clip compression method at level of T4-5. The study was conducted using 6 groups: control, trauma, trauma and solvent, trauma and resveratrol, trauma and quercetin, and trauma with combined resveratrol and quercetin. All rats were euthanized 48 hours after the procedure. Effects of resveratrol and quercetin on serum and tissue total antioxidant capacity and paraoxanase activity level were examined.
RESULTS: Compared to trauma group, there was a significant increase in total antioxidant capacity and paraoxanase activity level in resveratrol, quercetin, and combined treatment groups. There was no significant difference between resveratrol and quercetin groups with regard to total antioxidant capacity and paraoxanase activity level. Total antioxidant capacity and paraoxanase activity level were significantly higher in solvent group than trauma group. In histopathological evaluation, there was a decrease in polymorphonuclear leukocyte infiltration in solvent, resveratrol, quercetin, and combined treatment groups.
CONCLUSION: Biochemical and histological staining results of present study showed that resveratrol and quercetin may be effective in preventing secondary damage in spinal cord injury.

Keywords: Antioxidant, experimental spinal cord injury, quercetin, resveratrol.

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