Ulus Travma Acil Cerrahi Derg. 2023; 29(12): 1329-1334 | DOI: 10.14744/tjtes.2023.60621

Deneysel omurilik yaralanmasý olan sýçanlarda Shilajit'in etkisinin histopatolojik ve immünohistokimyasal olarak incelenmesi

Eyüp Çetin¹, Tunahan Samcak², Ömer Faruk Keleþ³, Ýlker Ünlü⁴, Mehmet Edip Akyol⁵, Özkan Arabacý^5
1Saðlýk Bilimleri Üniversitesi Haydarpaþa Numune Eðitim ve Araþtýrma Hastanesi, Beyin ve Sinir Cerrahisi Anabilim Dalý, Ýstanbul
²Sivas Cumhuriyet Üniversitesi, Veteriner Fakültesi, Cerrahi Anabilim Dalý, Sivas
³Van Yüzüncü Yýl Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalý, Van
⁴Ýstanbul Esenyurt Üniversitesi, Saðlýk Meslek Yüksek Okulu, Beyin ve Sinir Cerrahisi Bölümü, Ýstanbul
⁵Van Yüzüncü Yýl Üniversitesi Týp Fakültesi, Beyin ve Sinir Cerrahisi Anabilim Dalý, Van

AMAÇ: Bu çalýþma, deneysel olarak omurilik yaralanmasý (SCI) oluþturulan sýçanlarda Shilajit'in histopatolojik ve immünohistokimyasal etkilerini araþtýrmak üzere tasarlandý.
GEREÇ VE YÖNTEM: Sýçanlar üç gruba ayrýldý. Kontrol grubu: Spinal kord hasarý oluþturulan fakat herhangi bir ilaç uygulanmayan gruptur. Düþük doz grubu: Spinal kord hasarý oluþturulduktan sonra 150 mg/kg dozunda ameliyat sonrasý 1. saatte, 1. günde 2. günde ve 3. günde intraperitoneal Shilajit verilen gruptur. Yüksek doz grubu: Spinal kord hasarý oluþturulduktan sonra 250 mg/kg dozunda ameliyat sonrasý 1. saatte, 1. günde 2. günde ve 3. günde intraperitoneal Shilajit verilen gruptur. Ötenazi sonrasýnda spinal kordan alýnan ince kesitler ise histopatolojik ve immünohistokimyasal incelemeye gönderildi.
BULGULAR: Yüksek doz grubunun histopatolojik incelemesinin morfolojik bulgular açýsýndan düþük doz grubu ve kontrol grubuna kýyasla daha düþük miktarlarda olduðu gözlendi. Spinal hasarlý sýçanlarýn kontrol grubunda belirgin derecede CD68 immün reaksiyonu gözlenirken, Shilajit uygulanan gruplarda ise pozitif immün reaksiyonun anlamlý düzeyde azaldýðý saptandý.
SONUÇ: Omurilik yaralanmasýnda Shilajit kullanýlmasýnýn sekonder hasarýn etkilerini azaltacaðý ve bu hastalarda tedavi olarak verilmesinin sonuçlar üzerinde olumlu etkiler yapacaðýný düþünmekteyiz.

Anahtar Kelimeler: Spinal kord yaralanmasý, shilajit, omurilik felci, deneysel çalýþma, primer hasarlanma, sekonder hasarlanma.

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Histopathological and immunohistochemical investigation of the effect of Shilajit in rats with experimental spinal cord injury

Eyüp Çetin¹, Tunahan Samcak², Ömer Faruk Keleþ³, Ýlker Ünlü⁴, Mehmet Edip Akyol⁵, Özkan Arabacý^5
1Department of Neurosurgery, Health Sciences University Haydarpaþa Numune Training and Research Hospital, Ýstanbul-Türkiye
²Department of Surgery, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, Sivas-Türkiye
³Department of Patology, Faculty of Veterinary Medicine, Van Yüzüncü Yýl University, Van-Türkiye
⁴Department of Neurosurgery, Faculty of Health Sciences, Ýstanbul Esenyurt University, Ýstanbul-Türkiye
⁵Department of Neurosurgery, Faculty of Medicine, Van Yüzüncü Yýl University, Van-Türkiye

BACKGROUND: This experimental study was designed to investigate the histopathological and immunohistochemical effects of Shilajit in rats with experimentally induced spinal cord injury (SCI).
METHODS: The rats were divided into three groups: Control group: The group in which spinal cord damage was created but no drug was administered. Low-dose group: This is the group in which intraperitoneal Shilajit is given at a dose of 150 mg/kg at the 1st h, 1st day, 2nd day, and 3rd day after spinal cord damage was induced. High-dose group: This is the group in which intraperitoneal Shilajit is given at a dose of 250 mg/kg at the 1st h, 1st day, 2nd day, and 3rd day after spinal cord damage was induced. Thin sections taken from the spinal cord after euthanasia were sent for histopathological and immunohistochemical examination.
RESULTS: Histopathological examination of the high-dose group showed lower amounts of morphological findings compared to the low-dose group and control group. While a significant CD68 immune reaction was observed in the control group of rats with spinal injury, the positive immune reaction was found to be significantly decreased in the Shilajit-applied groups.
CONCLUSION: It is thought that the use of Shilajit in SCI will reduce the effects of secondary damage in SCI and that its administra-tion to such patients will have positive effects on the results.

Keywords: Experimental study, primary injury, secondary injury, shilajit; spinal cord paralysis; spinal cord injury.

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