EFFECTS OF GRANULOCYTE COLONY STIMULATING FACTOR ON BACTERIAL TRANSLOCATION DUE TO BURN INJURY

Certain defects of cellular and humoral immunity have been established in burn patients. Likewise translocation of enteric bacteria to mezenteric lymph nodes and to distant organs has been observed following serious thermal injury and this translocation has shown relationship with burn mortality. Bearing this factors in mind effects of Granulocyte Colony Stimulating Factor (G-CSF), a leukocyte function improving drug on bacterial translocation has been investigated. 24 Wistar Albino rats weighing 250gr have been scalded 30% and the lesion infected by 1x103 cfu Pseudomonas Aeruginosa. The control group (n-12) has been treated with 5% Dextroz solution subcutaneously starting two days preburn, and the study group (n-12) with 100ug/kg human G-CSF (Neupogen - Roche) subcutaneously. On the 4th post burn day translocation to mesenteric lymph nodes (MLN), liver and spleen has been investigated. Translocation in the control group was 12/12 in MLN, 12/12 in liver an 12/12 in spleen where as in the study group the values were 5/12 in MLN (p<0.01), 6/12 in liver (p<0.01) and 6/12 in spleen (p<0.01). Translocation values in MLN were 986 349 cfu/gr (p<0.05) in the study group. It has been observed that G-CSF is significantly effective in reducing bacterial translocation due to burn injury infection.

YANIÐA BAÐLI BAKTERÝYEL TRANSLOKASYONA GRANÜLOSÝT KOLONÝ STÝMÜLAN FAKTÖRÜN ETKÝLERÝ

Yanýklý hastalarda hücresel ve hümoral immun fonksiyonlarda birtakým defektler tesbit edilmiþtir. Keza ciddi termal yaralanma sonucu enterik bakterilerin mezenter lenf bezleri ve uzak organlara translokasyon oluþturduðu gözlenmiþ ve translokasyon ile yanýk mortalitesi arasýnda iliþki gözlenmiþtir. Bu bulgulara dayanarak lökositlerin fonksiyonlarýný arttýrýcý bir ilaç olan Granülosit Koloni Stimulan Faktör'ün (G-CSF) bakteriyel translokasyona etkisi araþtýrýldý. 24 adet 250gr erkek Wistar Albino rata %30 haþlanma yanýðý ve 1x10³ koloni oluþturan ünite (cfu) Pseudomonas Aeruginosa ile enfeksiyon oluþturuldu. Kontrol grubuna (12 adet), yanýktan 2 gün önce baþlayarak %5 Dextroz, tedavi grubuna (12 adet) ise 100mg/kg dozda recombinant human G-CSF (Nuepogen-Roche) günde tek doz. subkutan uygulandý. Yanýk sonrasý 4. gün raflarda mezenter lenf ganglionlarý (MLG), karaciðer ve dalaða translokasyon araþtýrýldý. Kontrol grubunda MLG'da (5/12p<0.01), karaciðerde (6/12p<0.01) ve dalakta (6/12p<0.01) translokasyon oluþtu. MLG'deki translokasyon miktarý kontrol grubunda 986 349 cfu/1 gr tedavi grubunda 371 151 cfu/gr (p<0.05) bulundu. G-CSF'nin yanýk ve infeksiyona baðlý bakteriyel translokasyonu anlamlý derecede azalttýðý tesbit edilmiþtir.