Ulus Travma Acil Cerrahi Derg. 2008; 14(4): 277-283

Hemorajik þok oluþturulan köpeklerde beyin hasarý ve serum nitrik oksit seviyelerine pirasetamýn etkisi

Seda Özkan¹, Ýbrahim Ýkizceli¹, Erdoðan Mütevelli Sözüer², Levent Avþaroðullarý¹, Figen Öztürk³, Sebahattin Muhtaroðlu⁴, Okhan Akdur¹, Can Küçük², Polat Durukan^4
1Erciyes Üniversitesi Týp Fakültesi Acil Týp Anabilim Dalý, Kayseri
²Erciyes Üniversitesi Týp Fakültesi Genel Cerrahi Anabilim Dalý, Kayseri
³Erciyes Üniversitesi Týp Fakültesi Patoloji Anabilim Dalý, Kayseri
⁴Erciyes Üniversitesi Týp Fakültesi Biyokimya Anabilim Dalý, Kayseri

AMAÇ: Hemorajik þok oluþturulan köpeklerde, pirasetamýn þoka baðlý nitrik oksit düzeylerinde deðiþikliðe ve beyin dokusunda oluþan hipoksiye etkisinin olup olmadýðý araþtýrýldý. GEREÇ-YÖNTEM: Denekler randomize olarak 10’ar köpekten oluþan dört gruba ayrýldý. Grup I, bir saatlik hemorajik þoka uðratýldý ve hemorajik þok sonrasý tedavi verilmedi. Grup II, bir saatlik hemorajik þoka uðratýldý ve þok sonrasý kan ile birlikte serum fizyolojik verildi. Grup III, bir saat hemorajik þoka uðratýldý ve þok sonrasý kan ile birlikte intravenöz pirasetam verildi. Grup IV’e ise þok ve tedavi uygulanmadý. Deneyin baþlangýcýnda, 60., 120. ve 180. dakikalarda nitrik oksit analizi için kan örnekleri alýndý. Deneyin sonunda, histopatolojik inceleme için beyin doku örnekleri çýkarýldý. BULGULAR: Kan basýncý ve nabýz deðerlerindeki düzelmenin Grup III’deki deneklerde Grup II’deki deneklere göre daha iyi olduðu saptandý. Grup I’de nitrik oksit seviyelerinde yükselme olmasýna raðmen, pirasetamla nitrik oksit seviyeleri arasýnda herhangi bir iliþki saptanmadý. Deneyin sonunda, Grup III’de hemorajik þoka baðlý geliþen beyin hasarýndaki düzelmenin kontrol grubuna göre daha iyi olduðu saptandý. SONUÇ: Tedaviye eklenen pirasetam hemorajik þokta iskemik hasarý azaltabilir.

Anahtar Kelimeler: Beyin hasarý, köpek; deneysel model; hemorajik; nitrik oksit; pirasetam; þok; tedavi.

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The effect of piracetam on brain damage and serum nitric oxide levels in dogs submitted to hemorrhagic shock

Seda Özkan¹, Ýbrahim Ýkizceli¹, Erdoðan Mütevelli Sözüer², Levent Avþaroðullarý¹, Figen Öztürk³, Sebahattin Muhtaroðlu⁴, Okhan Akdur¹, Can Küçük², Polat Durukan^4
1Department Of Emergency Medicine, Medicine Faculty Of Erciyes University, Kayseri, Turkey
²Department Of General Surgery, Medicine Faculty Of Erciyes University, Kayseri, Turkey
³Department Of Pathology, Medicine Faculty Of Erciyes University, Kayseri, Turkey
⁴Department Of Biochemistry, Medicine Faculty Of Erciyes University, Kayseri, Turkey

BACKGROUND: To demonstrate the effect of piracetam on changes in brain tissue and serum nitric oxide levels in dogs submitted to hemorrhagic shock. METHODS: The subjects were randomized into four subgroups each consisting of 10 dogs. Hemorrhagic shock was induced in Group I for 1 hour and no treatment was given to this group. Blood and saline solutions were administered to Group II following 1 hour hemorrhagic shock. Blood and piracetam were given to Group III following 1 hour shock. No shock was induced and no treatment was applied to Group IV. Blood samples were obtained at the onset of the experiment and at 60, 120 and 180 minutes for nitric oxide analysis. For histopathological examination, brain tissue samples were obtained at the end of the experiment. RESULTS: The observed improvement in blood pressure and pulse rates in Group III was more than in Group II. Nitric oxide levels were increased in Group I; however, no correlation between piracetam and nitric oxide levels was determined. It was seen that recovery in brain damage in Group III was greater than in the control group. CONCLUSION: Piracetam, added to the treatment, may decrease ischemic damage in hemorrhagic shock.

Keywords: Brain damage, dogs; experimental model; hemorrhagic; nitric oxide; piracetam; shock; treatment.

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