Ulus Travma Acil Cerrahi Derg. 2009; 15(5): 416-422

Melatonin ve fosfolipidin sýçanlarda deneysel peritoneal adezyon oluþumu üzerine etkisi ve vasküler endotelyal büyüme faktörü ile iliþkisi

Yucel Aritas¹, Alper Akcan¹, Ali Riza Erdogan¹, Hulya Akgun², Recep Saraymen³, Hizir Akyildiz^1
1Erciyes Üniversitesi Týp Fakültesi Genel Cerrahi Anabilim Dalý, Kayseri
²Erciyes Üniversitesi Týp Fakültesi Patoloji Anabilim Dalý, Kayseri
³Erciyes Üniversitesi Týp Fakültesi Biyokimya Anabilim Dalý, Kayseri

AMAÇ
Deneysel adezyon formasyonu oluþturulan sýçanlarda, melatonin ve fosfolipidin adezyon geliþimine etkilerinin vasküler endotelyal büyüme faktörü (VEGF) ekspresyonu ile iliþkisi araþtýrýldý.
GEREÇ VE YÖNTEM
Çalýþmada 60 adet Wistar-Albino cinsi sýçan, rastgele 15’erlik sham, kontrol ve iki adet çalýþma gruplarýna ayrýldý. Sham grubuna sadece laparotomi yapýldý. Kontrol grubu ve çalýþma gruplarýnda sað alt kadranda pariyetal peritonda abrazyon yapýldý, çekum, ileum ve uterusun sað boynuzunda serozal defekt oluþturuldu; kontrol grubunda periton boþluðuna Ringer laktat verildi. Çalýþma gruplarýndan birine melatonin, diðerine fosfolipid süspansiyonu verildi. Bütün gruplarda 15 gün sonra relaparotomi yapýlarak, adezyon geliþimi deðerlendirilip skorlama yapýldý.
BULGULAR
Kontrol grubuna göre sham, melatonin ve fosfolipid gruplarýnda adezyon geliþimi belirgin olarak düþüktü (p<0,05). Kontrol grubunda yapýþýklýk olan alanlarda VEGF boyamasý diðer gruplara göre anlamlý derecede yüksekti (p<0,05). Melatonin ve fosfolipid grubunda VEGF boyamasý ile normal alanlardaki VEGF boyamasý arasýnda anlamlý fark yoktu (p>0,05).
SONUÇ
Deneysel adezyon modeli oluþturulan sýçanlarda melatonin ve fosfolipid adezyon geliþimini azaltmaktadýr. Geliþen adezyonun þiddeti ile VEGF ekspresyonu arasýnda paralellik vardý. Þiddetli adezyon geliþen alanlarda VEGF ekspresyonu artmýþtý.

Anahtar Kelimeler: Melatonin, peritoneal adezyon; fosfolipid; vasküler endotelyal büyüme faktörü.

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Effects of melatonin and phospholipid on adhesion formation and correlation with vascular endothelial growth factor expression in rats

Yucel Aritas¹, Alper Akcan¹, Ali Riza Erdogan¹, Hulya Akgun², Recep Saraymen³, Hizir Akyildiz^1
1Department Of General Surgery, University Of Erciyes, School Of Medicine, Kayseri, Turkey
²Department Of Pathology, University Of Erciyes, School Of Medicine, Kayseri, Turkey
³Department Of Biochemistry, University Of Erciyes, School Of Medicine, Kayseri, Turkey

BACKGROUND
The aim of this experimental study was to investigate the effects of melatonin and phospholipid on adhesion formation and the correlation with vascular endothelial growth factor (VEGF) expression in rats.
METHODS
Sixty Wistar-Albino rats were divided into four groups as sham, control and two study groups, each including 15 rats. In the sham group, laparotomy was the only procedure. Left lower parietal peritoneum was abraded after laparotomy and serosal defects formed on the cecum, ileum and right uterine horn in the study and control groups. Ringer lactate was then applied to the control group, while melatonin and phospholipid suspension were applied separately in the two study groups. Relaparotomy was performed in all groups on the 15th day to score and evaluate the adhesion formation.
RESULTS
Adhesion formation was significantly lower in the sham, melatonin and phospholipid groups than in the control group (p<0.05). VEGF staining was significantly higher in the control group with adhesion areas compared to the other groups (p<0.05). When VEGF staining was compared, there was no significant difference between VEGF- stained and normal areas in the melatonin and phospholipid groups.
CONCLUSION
Melatonin and phospholipid decreased the adhesion formation in an experimental adhesion model in rats. There is a correlation between adhesion severity and VEGF expression.

Keywords: Melatonin, peritoneal adhesion; phospholipid; vascular endothelial growth factor.

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ATIF KOPYALA