THE EFFECTS OF LEVAMIZOL ON INTESTINAL ANASTOMOSIS IN TRANSFUSED GUINEA

In this experimental study, the effects of an antihelmintic and immunostimulating agent (drug) levamizol on anastomosis recovery in blood transfused and normal Guinea pigs were investigated. A total of 56 animals were divided into 4 groups of each consisted of 14 subjects. An additional 10 Guinea pigs were employed for blood transfusion. Intestinal anastomosis were performed on all subjects. Following postoperative treatments were exercised: Group A: None (control group), Group B: Blood transfusion, Group C: Intraabdominal levamizol, Group D: Transfusion+ Levamizol after recording the mortality rate of each group, relaparotomy was applied on 50% the subjects on the 3rd postoperative day, and on the remaining 50% of the subjects on the 7th postoperative day. Findings on intraabdominal sepsis, resistance of anastomosis, explosion pressure were evaluated. Statistical comparision of groups were accomplished by the variance analysis. In the transfusion group an increase in the sepsis findings were determined and the blowing pressure was found to be meaningfully lower than the control group. Intraabdominal levamizol application f allowing the blood transfusion was observed to reduce the mortality by diminishing the intraabdominal sepsis and anastomosis abscess, and to increase the anastomosis pressure and recovery (p<0.01). The histopathological recovery in levamizol administered groups were found to be better than the group received only blood transfusion. Levamizol application without blood transfusion resulted in equal mortality and morbidity to that of control group.

KAN TRANSFÜZYONU YAPILAN KOBAYLARDA LEVAMÝZOLÜN ÝNTESTÝNAL ANASTOMOZ ÜZERÝNE ETKÝLERÝ

Bu deneysel çalýþmada bir antihelmintik ve immünostimülatör olan levamizolün normal ve kan transfüzyonu yapýlmýþ kobaylarda anastomoz iyileþmesi üzerine etkilerini araþtýrmak amacýyla, her grupta 14 kobay (Guinea Pig) olmak üzere 4 grup halinde toplam 56 kobay ve kan transfüzyonu için de 10 adet kobay kullanýldý. Tüm kobaylarda intestinal anastomoz uygulandý. Grup A: Kontrol grubu, Grub B: Kan transfüzyonu yapýlan grup, Grup C: Batýniçi levamizol uygulanan grup, Grup D: Transfüzyon ve levamizol uygulanan grup. Deney gruplarýnda mortalite hýzý kaydedildikten sonra deneklerin yarýsýna postoperatif 3. günde ve diðer yarýsýna 7. günde olmak üzere relaparotomi uygulandý. Ýntraabdominal sepsis bulgularý, anastomoz dirençleri, patlama basýnçlarý deðerlendirildi. Gruplar arasýndaki istatistiksel karþýlaþtýrma "Varyans Analiz" testi ile yapýldý. Transfüzyon grubunda sepsis bulgularý arttý ve patlama basýncý kontrol grubuna göre anlamlý olarak düþük bulundu. Kan transfüzyonundan sonra intraabdominal verilen levamizolün intraabdominal sepsisi ve anastomoz absesini azaltarak mortaliteyi düþürdüðü, ayrýca anastomoz iyileþmesi ve anastomoz direncini anlamlý alarak (p<0.01) arttýrdýðý gözlendi. Levamizol uygulanan gruplarda histopatolojik iyileþme sadece transfüzyon uygulanan gruba göre daha iyi bulundu. Levamizolün transfüzyon olmadan verildiðinde ise kontrol grubu ile eþdeðerde mortalite ve morbiditiye sahip olduðu bulundu.