Ulus Travma Acil Cerrahi Derg. 2020; 26(5): 663-670 | DOI: 10.14744/tjtes.2019.45570

The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis

Yuksel Altinel¹, Þenay Yalçýn², Gulçin Ercan¹, Erkan Yavuz¹, Candaþ Erçetin¹, Osman Bilgin Gülçiçek¹, Atilla Çelik¹, Güven Özkaya³, Hafize Uzun^4
1Department of General Surgery, Baðcýlar Training and Research Hospital, Ýstanbul-Turkey
²Department of Pathology, Baðcýlar Training and Research Hospital, Ýstanbul-Turkey
³Department of Biostatistics, Uludað University Faculty of Medicine, Bursa-Turkey
⁴Department of Biochemistry, Cerrahpaþa University Faculty of Medicine, Ýstanbul-Turkey

BACKGROUND: Curcumin is an antioxidant and anti-inflammatory molecule known to be a potent inhibitor of nuclear factor kappa B (NF-kappa B). In this study, we aimed to investigate the therapeutic effects of curcumin on colitis induced by a 2,4,6-trinitrobenzene sulfonic acid (TNBS).
METHODS: After the induction of colitis under anesthesia, 42 rats were divided into six groups as follows; the curcumin oral group, curcumin (20 mg/kg); the corn oil oral group, corn oil (20 mg/kg) using gastric gavage, the curcumin rectal group, curcumin; the corn oil rectal group, corn oil; the control group, 1 mL saline solution (0.9% NaCl) were administered using the rectal route. In the sham group, only rectal catheterization was performed. At the end of the seven day, the blood and intestinal tissue samples were obtained for histopathological examination and for MPO, MDA, NO, PDGF, IL-6, TNF-alpha, NF-kappaB.
RESULTS: The macroscopic damage score was significantly higher in curcumin oral, corn oil oral and saline groups when compared to the sham group (p<0.05). The significant differences between groups were evaluated using the biochemical analysis of intestinal tissue for IL-6, NO, NF-κB, PDGF, TNF-α, MDA, MPO (p<0.05). NF-κB levels of blood in curcumin oral, curcumin rectal, sham, corn oil oral, corn oil rectal groups were significantly increased when compared to saline rectal group (p≤0.001). NF-κB serum levels of corn oil rectal and control group (p≤0.001) were lower than the sham group (p=0.012).
CONCLUSION: The effects of curcumin improved possibly by modulating the NF-κB signaling pathway should be considered against colitis alone or in combination with the conventional anti-colitic therapies in future studies.

Keywords: Anti-inflammatory, anti-oxidant; colitis; curcumin; NF-kappaB.

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Kurkumin’in PDGF ekspresyonu ve NF-kappa B üzerindeki etkisi: TNBS kolit modeli

Yuksel Altinel¹, Þenay Yalçýn², Gulçin Ercan¹, Erkan Yavuz¹, Candaþ Erçetin¹, Osman Bilgin Gülçiçek¹, Atilla Çelik¹, Güven Özkaya³, Hafize Uzun^4
1Baðcýlar Eðitim ve Araþtýrma Hastanesi, Genel Cerrahi Kliniði, Ýstanbul
²Baðcýlar Eðitim ve Araþtýrma Hastanesi, Patoloji Kliniði, Ýstanbul
³Uludað Üniversitesi Týp Fakültesi, Biyoistatistik Anabilim Dalý, Bursa
⁴Cerrahpaþa Üniversitesi Týp Fakültesi, Biyokimya Anabilim Dalý, Ýstanbul

AMAÇ: Kurkumin, nükleer faktör kappa B’nin (NF-kappa B) güçlü bir inhibitörü olduðu bilinen antioksidan ve antienflamatuvar bir moleküldür. Kurkumin’in 2,4,6-trinitrobenzen sülfonik asit (TNBS) ile indüklenen kolit modeli üzerindeki etkisini araþtýrmayý amaçladýk.
GEREÇ VE YÖNTEM: Anestezi altýnda kolit indüksiyonundan sonra, 42 sýçan altý gruba ayrýldý; kurkumin oral grup, kurkumin (20 mg/kg); mýsýr yaðý oral grubu, mýsýr yaðý (20 mg/kg) mide gavajý yoluyla, kurkumin rektal grubu, kurkumin; mýsýr yaðý rektal grubu, mýsýr yaðý. Kontrol grubuna 1 mL salin solüsyonu (%0.9 NaCl) rektal yolla uygulandý. Sham grubunda sadece rektal kateterizasyon yapýldý. Yedi gün sonunda, histopatolojik inceleme ve MPO, MDA, NO, PDGF, IL-6, TNF-alfa, NF-kappa B için kan ve baðýrsak doku örnekleri alýndý.
BULGULAR: Makroskobik hasar skoru, kurkumin oral, mýsýr yaðý oral ve salin gruplarýnda sham grubuna göre anlamlý olarak yüksek bulundu (p<0.05). Gruplar arasýndaki anlamlý fark, IL-6, NO, NF-kB, PDGF, TNF-α, MDA, MPO intestinal dokunun biyokimyasal analizinde deðerlendirildi (p<0.05). Kurkumin oral, kurkumin rektal, sham, mýsýr yaðý oral, mýsýr yaðý rektal gruplarýnda NF-kB kan seviyeleri, salin rektal grubuna göre anlamlý olarak arttý (p=0.001). Mýsýr yaðý rektal ve kontrol grubunun NF-kB serum seviyeleri (p≤0.001) sham grubundan düþük bulundu (p=0.012).
TARTIÞMA: Kurkumin’in, NF-kB sinyal yolundaki duzenleyici etkisi muhtemelen iyileþtirici özelliði ile gelecekteki çalýþmalarda tek baþýna kolite karþý veya konvansiyonel anti-kolitik tedaviler de kombinasyon halinde düþünülmelidir.

Anahtar Kelimeler: Antienflamatuvar, anti-oksidan; kolit; kurkumin; NF-kB.

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