Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells

BACKGROUND: The autologous bone grafts still have been used as the gold standard to initiate and facilitate bone healing in cases with bone defects. Because of some disadvantages of autologous bone grafts, the new biocomposite grafts have been researched. The purpose of the present study was to investigate whether the bone marrow-derived mesenchymal stem cells (BM-MSCs) loaded into a biocomposite scaffold enhance bone regeneration.
METHODS: In our study, a 10 mm osteoperiosteal segmental bone defect was created in the middle of the right and left ulnar bones of eight rabbits. The created defects were filled in the right ulnar bones of eight rabbits (Group I) with BM-MSCs loaded onto a bio-composite scaffold (Plexur PTM, Osteotech, Eatontown, NJ, USA) and in the other ulnar bones of the same rabbits (Group II) with only biocomposite graft. Radiographs of each forelimb were taken postoperatively at the end of the 6th week. Then, the rabbits were euthanized pharmacologically for histopathological evaluation.
RESULTS: Were scored using a modified Lane and Sandhu scoring system. All defects healed in both groups. Radiological and histo-logical total scores were slightly better in Group I, but statistical tests did not reveal any significant differences between the two groups at the end of the 6th week radiologically and histologically (p>0.05).
CONCLUSION: The results of our study demonstrated that in rabbit ulnar segmental bone defect model was obtained satisfactory regeneration with using biocomposite graft with or without BM-MSCs.

Kýsmi yük taþýyan tavþan ulnar segmental defektlerin biyouyumlu greftler ve/veya kemik iliðinden türetilmiþ mezenkimal kök hücreler ile yenilenir

AMAÇ: Otolog kemik greftleri, kemik defekti olan olgularda kemik iyileþmesini baþlatmak ve kolaylaþtýrmak için altýn standart olarak hala kullanýl-maktadýr. Otolog kemik greftlerinin bazý dezavantajlarý nedeniyle yeni biyokompozit greftler araþtýrýlmýþtýr. Bu çalýþmanýn amacý, bir biyokompozit iskeleye yüklenen kemik iliðinden türetilen mezenkimal kök hücrelerin (BM-MSC’ler) kemik rejenerasyonunu artýrýp artýrmadýðýný araþtýrmaktý. GEREÇ VE YÖNTEM: Çalýþmamýzda sekiz adet tavþanýn sað ve sol ulnar kemiðin orta kýsmýnda 10 mm’lik osteoperiosteal segmental kemik defekti oluþturuldu. Tavþanlarýn sað ulnar defektleri (Grup 1) bir biyokompozit iskeleye (Plexur PTM, Osteotech, Eatontown, NJ, ABD) yüklenmiþ BM-MSC’ler ile tedavi edilirken, ayný tavþanlarýn sol ulnar kemiklerine (grup II) sadece biyokompozit greft ile tedavi uygulandý. Altýncý haftanýn sonunda ameliyat sonrasý olarak her bir ön ayaðýn radyografileri çekildi. Daha sonra tavþanlara histopatolojik deðerlendirme için farmakolojik olarak ötenazi yapýldý. Sonuçlar, modifiye Lane ve Sandhu puanlama sistemi kullanýlarak puanlandý.
BULGULAR: Her iki grupta da tüm defektler iyileþti. Radyolojik ve histolojik toplam skorlar grup I’de daha iyiydi, ancak istatistiksel olarak altýncý haftanýn sonunda radyolojik ve histolojik olarak iki grup arasýnda anlamlý bir farklýlýk gözlenmedi (p>0.05).
TARTIÞMA: Çalýþmamýzýn sonuçlarý, tavþan ulnar segmental kemik defekt modelinin, BM-MSC’ler içeren veya içermeyen biyokompozit greft kullanýlarak tatmin edici bir rejenerasyon elde edildiðini göstermiþtir.