Ulus Travma Acil Cerrahi Derg. 2011; 17(5): 383-389 | DOI: 10.5505/tjtes.2011.99835

Sýçanlarda seruleinle deneysel olarak oluþturulan akut pankreatitte alfa lipoik asitin faydalý etkileri

Nuriye Esen Bulut¹, Erkan Özkan², Osman Ekinci³, Ender Dulundu², Ümit Topaloðlu², Ahmet Özer Þehirli⁴, Feriha Ercan⁵, Göksel Þener^4
1Fatih Sultan Mehmet Eðitim Ve Araþtýrma Hastanesi, Genel Cerrahi Kliniði, Ýstanbul
²Haydarpaþa Numune Eðitim Ve Araþtýrma Hastanesi,5. Genel Cerrahi Kliniði, Ýstanbul
³Haydarpaþa Numune Eðitim Ve Araþtýrma Hastanesi, Anestezi Ve Reanimasyon Kliniði, Ýstanbul
⁴Marmara Üniversitesi Eczacýlýk Fakültesi, Farmakoloji Ana Bilim Dalý, Ýstanbul
⁵Marmara Üniversitesi Týp Fakültesi, Histoloji Ve Embriyoloji Ana Bilim Dalý, Ýstanbul

AMAÇ
Bu çalýþmada, sýçanlarda seruleinle deneysel olarak oluþturulan akut pankreatit (AP) modelinde alfa lipoik asitin (ALA) kan biyokimyasal parametreleri ve doku düzeyindeki etkileri araþtýrýldý.
GEREÇ VE YÖNTEM
Her biri 8 sýçandan oluþan 3 grup oluþturuldu. Grup 1: Kontrol; Grup 2: Seruleinle pankreatit oluþturulup salin verilen grup; Grup 3: Seruleinle pankreatit oluþturulup ALA verilen grup. AP intraperitoneal olarak 20 µg/kg dozunda seruleinin 1’er saat aralýklarla 4 defa verilmesiyle oluþturuldu. Son serulein dozundan 12 saat sonra hayvanlar dekapite edildi. Kan amilaz, lipaz, interlökin (IL)-1ß, tümör nekroz faktör (TNF)-alfa düzeyleri, pankreas doku glutatyon (GSH), malondialdehid (MDA), miyeloperoksidaz (MPO) ve Na+-K+-ATPase aktivitesi belirlendi. Ayrýca pankreas doku örnekleri histopatolojik olarak mikroskopta deðerlendirildi.
BULGULAR
Seruleinle pankreatit oluþturulan grupta kan amilaz, lipaz, IL-1ß, TNF-alfa düzeyleri, doku MDA, MPO düzeyleri anlamlý derecede artarken, doku GSH ve Na+-K+-ATPase aktivitesi anlamlý derecede azaldý. ALA tedavisiyle bu deðerlerde ve histopatolojide tersine deðiþiklikler, düzelme ve iyileþme görüldü.
SONUÇ
Mevcut bulgular ALA tedavisinin, pankreasta oluþabilecek serbest radikallerin neden olduðu organ ve fonksiyon bozukluðunu önleyerek morbidite ve mortaliteyi önemli oranda azaltabileceðini düþündürmektedir.

Anahtar Kelimeler: Akut pankreatit, alfa lipoik asit; serulein; sitokinler; serbest oksijen radikalleri.

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Beneficial effects of alpha lipoic acid on cerulein-induced experimental acute pancreatitis in rats

Nuriye Esen Bulut¹, Erkan Özkan², Osman Ekinci³, Ender Dulundu², Ümit Topaloðlu², Ahmet Özer Þehirli⁴, Feriha Ercan⁵, Göksel Þener^4
1Fatih Sultan Mehmet Training And Research Hospital, General Surgery Clinic, Istanbul
²Haydarpasa Numune Training And Research Hospital,5 Th General Surgery Clinic, Istanbul
³Haydarpasa Numune Training And Research Hospital, Anesthesia And Reanimation Clinic, Istanbul
⁴Marmara University, Faculty Of Pharmacy, Department Of Pharmacology, Istanbul
⁵Marmara University, Faculty Of Medicine, Department Of Histology And Embryology, Istanbul

BACKGROUND
The present study aimed to determine the effects of alpha lipoic acid (ALA) on blood and tissue biochemical parameters, as well as tissue histopathology, in an experimental rat model of cerulein-induced acute pancreatitis (AP).
METHODS
Three groups consisting of eight rats each were used, as follows: Group 1, controls; Group 2, cerulein-induced pancreatitis group treated with saline; and Group 3, cerulein-induced pancreatitis group treated with ALA. AP was induced by intraperitoneal administration of cerulein (20 µg/kg) 4 times at 1-hour intervals. The animals were decapitated 12 hours after the last dose of cerulein. Blood amylase, lipase, interleukin (IL)-1ß, and tumor necrosis factor (TNF)-α levels, pancreas tissue glutathione (GSH) and malondialdehyde (MDA) levels, as well as myeloperoxidase (MPO) and Na+-K+-ATPase activity were measured. Pancreatic tissue samples were also evaluated histopathologically under a light microscope.
RESULTS
While plasma amylase, lipase, IL-1ß, and TNF-α levels, and tissue MDA and MPO levels significantly increased in rats with cerulean-induced AP, tissue GSH and Na+-K+-ATPase activity significantly reduced. These changes were reversed and improved with ALA treatment.
CONCLUSION
Our findings suggest that ALA may significantly reduce morbidity and mortality by preventing organ dysfunction induced by free radicals in the pancreas.

Keywords: Acute pancreatitis, alpha lipoic acid; cerulein; cytokines; free oxygen radicals.

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