Ulus Travma Acil Cerrahi Derg. 2013; 19(4): 294-298 | DOI: 10.5505/tjtes.2013.32458

Lornoksikamýn sýçanlarda diffüz travmatik beyin hasarýnda beyin ödemi ve kan beyin bariyeri üzerine etkileri

Ýsmet Topçu¹, Gül Gümüþer², Eda Bayram¹, Feray Aras², Ýsmail Çetin¹, Cüneyt Temiz³, Melek Çivi^1
1Celal Bayar Üniversitesi Týp Fakültesi, Anesteziyoloji Ve Yoðun Bakým Anabilim Dalý, Manisa
²Celal Bayar Üniversitesi Týp Fakültesi, Nükleer Týp Anabilim Dalý, Manisa
³Celal Bayar Üniversitesi Týp Fakültesi, Nöroþirürji Anabilim Dalý, Manisa

Amaç: Bu çalýþmada diffüz travmatik beyin hasarý (TBH) sonrasý, lornoksikamýn kan beyin bariyeri (KBB) ve beyin ödemi üzerine etkileri araþtýrýldý.
Gereç Ve Yöntem: Yirmi eriþkin erkek Wistar albino sýçana anestezi uygulamasý sonrasý Marmarou yöntemi ile deneysel kapalý kafa travmasý oluþturuldu. Kafa travmasý sonrasý sýçanlar randomize olarak iki gruba ayrýldý: Grup I intraperitoneal yolla 2 mL salin uygulanan kontrol grubu ve Grup II intraperitoneal yolla 2 mL 1.3 mg kg-1 lornoksikam verilen lornoksikam grubu. Kafa travmasýndan 24 saat sonra 99 mTc pentetate (DTPA) 37 MBq dozda verildi ve her bir sýçanýn posterior planar görüntüsü bir Infinia gama kamera kullanýlarak elde edildi. KBB permebilitesinin görüntülenmesi sonrasý beyin dokularý kranyumdan disseke edildi. Tüm örneklerin beyin su içeriði (BSI) ýslak-kuru metodu ile hesaplandý.
Bulgular: Grup I lesion/background (L/b) oranlarý erken dönem (5. dk) 3,76±0,46 ve geç dönem (60. dk) 3,02±0,66 idi. Grup II L/b oranlarý erken dönem 3,52±0,96, geç dönem 2,63±0,63 olarak saptandý (p>0,05). BSC Grup I’de %79,6±2,5 ve Grup II’de %77,5±1,1 idi (p<0,05).
Sonuç: Bu TBH sýçan modelinde lornoksikamýn beyin ödemini azalttýðý ancak KBB geçirgenliðini etkilemediði görülmüþtür.

Anahtar Kelimeler: Kan beyin bariyeri, beyin ödemi; lornoksikam; travmatik beyin hasarý.

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The effects of lornoxicam on brain edema and blood brain barrier following diffuse traumatic brain injury in rats

Ýsmet Topçu¹, Gül Gümüþer², Eda Bayram¹, Feray Aras², Ýsmail Çetin¹, Cüneyt Temiz³, Melek Çivi^1
1Celal Bayar University, Department Of Anesthesiology And Intensive Care, Manisa, Turkey
²Celal Bayar University, Department Of Nuclear Medicine, Manisa, Turkey
³Celal Bayar University, Department Of Neurosurgery, Manisa, Turkey

Background: In this experiment, the effects of lornoxicam on brain edema and the blood brain barrier (BBB) following diffuse traumatic brain injury (TBI) were studied.
Methods: Twenty adult male Wistar albino rats were anesthetized, and experimental closed head trauma was induced by the Marmarou method. After head injury, the rats were randomly divided into two groups: Group I was the control group, to which 2 ml saline was administered intraperitoneally, and Group II was the lornoxicam group, to which 2 ml 1.3 mg kg-1 lornoxicam was administered intraperitoneally. Twentyfour hours after head trauma, 99 mTc pentetate (DTPA) was injected at a dose of 37 MBq, and posterior planar images of each rat were obtained using an Infinia gamma camera. After imaging of BBB permeability, brain tissues were dissected from the cranium. The brain water content (BWC) of each sample was calculated using the wet-dry method.
Results: The lesion/background (L/b) ratio of Group I was 3.76±0.46 and 3.02±0.66 for early (5th min) and late (60th min) imaging, respectively. In Group II, the L/b ratios were 3.52±0.96 and 2.63±0.63 for early and late imaging, respectively (p>0.05). BWC was 79.6±2.5% and 77.5±1.1% for Groups I and II, respectively (p<0.05).
Conclusion: In this rat model of TBI, lornoxicam reduced brain edema but did not affect BBB permeability.

Keywords: Blood brain barrier, brain edema; lornoxicam; traumatic brain injury.

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