Deneysel spinal kord travmas�nda eritropoetin ve tadalafilin terap�tik etkinli�i

K�ko�lu, �a�r�; Delen, Emre; Arslanta�, Ali; Arslanta�, Didem; K�ko�lu, Burcu; �zbek, Z�ht�; Uslu, Sema; Ak�nc�, Ahmet Tolgay

Cilt : 30 Say� : 12 Y�l : 2024

30/12Gelecek Say� Ar�iv Kapaklar Pop�ler Makaleler

ICMJE COI Form

H�zl� Arama

Deneysel spinal kord travmas�nda eritropoetin ve tadalafilin terap�tik etkinli�i [Ulus Travma Acil Cerrahi Derg]

Ulus Travma Acil Cerrahi Derg. 2016; 22(6): 521-525 | DOI: 10.5505/tjtes.2016.37571

Deneysel spinal kord travmas�nda eritropoetin ve tadalafilin terap�tik etkinli�i

�a�r� K�ko�lu¹, Emre Delen², Ali Arslanta�³, Didem Arslanta�⁴, Burcu K�ko�lu⁵, Z�ht� �zbek³, Sema Uslu⁶, Ahmet Tolgay Ak�nc�⁷
¹Eski�ehir Yunus Emre Devlet Hastanesi, N�ro�ir�rji Klini�i, Eski�ehir
²Edirne Devlet Hastanesi, N�ro�ir�rji Klini�i, Edirne
³Eski�ehir Osmangazi �niversitesi, N�ro�ir�rji Ana Bilim Dal�, Eski�ehir
⁴Eski�ehir Osmangazi �niversitesi, Halk Sa�l�� Ana Bilim Dal�, Eski�ehir
⁵Eski�ehir Osmangazi �niversitesi, Aile Hekimli�i Ana Bilim Dal�, Eski�ehir
⁶Eski�ehir Osmangazi �niversitesi, Biokimya Ana Bilim Dal�, Eski�ehir
⁷Tokat Devlet Hastanesi, N�ro�ir�rji Klini�i, Tokat

AMA�: Bu �al��ma, spinal kord travmas� (SCI) olu�turulan s��anlarda eritropoetin ve tadalafilin etkinli�ini ara�t�rmak i�in yap�ld�.
GERE� VE Y�NTEM: Sprague-Dawley cinsi 35 adet s��an be� gruba ayr�ld�. Spinal kord travmas� T10 vertebraya laminektomi i�leminden sonra klip kompresyon y�ntemi uygulanarak ger�ekle�tirildi. Birinci grupta travma yap�lmad� ve normal biyokimyasal de�erler i�in kullan�ld�. �kinci grup ��z�c� grubu idi ve travma sonras� 1 cc serum fizyolojik verildi. ��nc� grup travma sonras�nda tek doz 2000 �/kg intraperiteneal yol ile eritropoetin verildi. D�rd�nc� gruba ise travma sonras�nda tek doz 2 mg/kg oragastrik yol ile tadalafil verildi. Be�inci gruba herhangi bir tedavi verici i�lem yap�lmadan sadece travma uyguland� ve travmadaki biyokimyasal de�erlerin tespiti i�in kullan�ld�. T�m denekler cerrahi i�lemden 48 saat sonra desakrafiliye edildi. Kan ve doku �rneklerinden malondialdehid (MDA) ve toplam antioksidan kapasite (TAOK) de�erleri �l��ld�.
BULGULAR: Eritropoetin verilen grupta di�er t�m gruplara g�re en d��k serum ve doku MDA de�erlerine ula��lm��t�r. T�m gruplara g�re serum TAOK de�erleri en y�ksek tadalafil verilen grupta bulunmu� iken doku TAOK de�erleri en y�ksek eritropoetin verilen grupta tespit edilmi�tir.
TARTI�MA: Tadalafil ve eritropoetinin dokudaki oksidatif stresi azaltarak spinal kord travmas�nda ikincil hasarlanmay� engelleyebilece�i sonucuna var�ld�.

Anahtar Kelimeler: Eritropoetin, n�roproteksiyon, spinal kord hasar�, tadalafil.

Therapeutic efficacy of tadalafil and eriythropoietin in experimental spinal cord injury

�a�r� K�ko�lu¹, Emre Delen², Ali Arslanta�³, Didem Arslanta�⁴, Burcu K�ko�lu⁵, Z�ht� �zbek³, Sema Uslu⁶, Ahmet Tolgay Ak�nc�⁷
¹Department Of Neurosurgery, Eski�ehir Yunus Emre State Hospital, Eski�ehir, Turkey
²Department Of Neurosurgery, Edirne State Hospital, Edirne, Turkey
³Department Of Neurosurgery, Eski�ehir Osmangazi University,eski�ehir, Turkey
⁴Department Of Public Health, Eski�ehir Osmangazi University, Eski�ehir, Turkey
⁵Department Of Family Medicine, Eski�ehir Osmangazi University, Eski�ehir, Turkey
⁶Department Of Biochemistry, Eskisehir Osmangazi University, Eski�ehir, Turkey
⁷Department Of Neurosurgery, Tokat State Hospital, Tokat, Turkey

BACKGROUND: This experimental study was an investigation of the efficacy of erythropoietin and tadalafil in rats with induced spinal cord injury (SCI).
METHODS: Thirty-five Sprague Dawley rats were distributed into 5 groups. First group was used for normal biochemical values. Spinal cord injury was induced in 4 remaining groups with clip compression technique after laminectomy process to T10 vertebra. Second group was designated solvent group and received 1 cc physiological serum after injury. Third group was medicated with intraperitoneal 2000 u/kg single dose erythropoietin after injury. Orogastric 2 mg/kg single dose tadalafil was administered to fourth group after injury. Fifth group did not receive any treatment and was used for biochemical values with injury. All subjects were sacrificed 48 hours after application. Malondialdehyde (MDA) and total antioxidant capacity (TAOC) values were evaluated using blood and tissue samples.
RESULTS: Lowest serum and tissue MDA values were found in group with erythropoietin intake. While highest serum TAOC values of all groups were seen in tadalafil group, highest tissue TAOC values were observed in group given erythropoietin.
CONCLUSION: It was concluded that by decreasing oxidative stress, tadalafil and erythropoietin can inhibit secondary damage in SCI.

Keywords: Erythropoietin, neuroprotection, spinal cord injury, tadalafil.

Sorumlu Yazar: �a�r� K�ko�lu, T�rkiye
Makale Dili: �ngilizce

ATIF KOPYALA

Tam Metin PDF At�f dosyas� indir RIS EndNote BibTex Medlars Procite Reference Manager Yazara e-posta g�nder Benzer makaleler PubMed Google Scholar