Ulus Travma Acil Cerrahi Derg. 2007; 13(2): 85-93

Deneysel Spinal Kord Hasarlanmasýnda Koenzim Q10’in Etkinliði

Alaeddin Kerimoðlu¹, Özgür Paþaoðlu², Güngör Kanbak³, Volkan Hancý⁴, Filiz Özdemir³, Metin Ant Atasoy^5
1Sarýkamýþ Asker Hastanesi, Nöroþirürji, Kars, Türkiye
²Osmangazi Üniversitesi Týp Fakültesi, Patoloji Anabilim Dalý, Eskiþehir, Türkiye
³Osmangazi Üniversitesi, Týp Fakültesi Biyokimya Anabilim Dalý, Eskiþehir, Türkiye
⁴Sarýkamýþ Asker Hastanesi, Anesteziyoloji Ve Reanimasyon, Kars, Türkiye
⁵Osmangazi Üniversitesi Týp Fakültesi, Nöroþirürji Anabilim Dalý, Eskiþehir, Türkiye

AMAÇ: Bu çalýþmada, metilprednizolon, koenzim Q10 ve metilprednizolonla birarada koenzim Q10 tedavilerinin deneysel spinal kord yaralanmasýndaki etkinlikleri karþýlaþtýrýldý. GEREÇ-YÖNTEM: Sprague-Dawley cinsi 32 erkek sýçan (200-250 gr) dört gruba ayrýldý. Spinal kord hasarlanmasý ekstradural olarak T4-5 seviyesine yerleþtirilen anevrizma klibi ile uygulandý. Travmanýn ardýndan, grup K’ya (kontrol grubu) soya yaðý, grup M’ye (metilprednizolon grubu) 30 mg.kg-1 ardýndan idamede saatte 5,4 mg.kg-1 dozunda metilprednizolon, grup Q’ya (koenzim Q10 grubu) 10 mg.kg-1 koenzim Q10, grup MQ’ya (metilprednizolon ve koenzim Q10 grubu) 30 mg.kg-1 ardýndan idamede saatte 5,4 mg.kg-1 dozunda metilprednizolon ile 10 mg.kg-1 koenzim Q10 intraperitoneal olarak verildi. Travmadan 24 saat sonra sýçanlarýn spinal kord örnekleri alýnarak histopatolojik ve biyokimyasal inceleme yapýldý. BULGULAR: Histopatolojik incelemelerde grup K’da grup M, grup Q ve grup MQ’ya göre ödem þiddeti anlamlý olarak yüksekti (p<0,001). Grup M, grup Q ve grup MQ arasýnda ödem ve kanama açýsýndan anlamlý farklýlýk yoktu (p>0,05). Ortalama süperoksit dismutaz deðerleri diðer gruplarýn tümünde grup K’ya göre, grup MQ’de grup M’ye göre anlamlý olarak daha düþüktü (p<0,05). Malonildialdehidin ortalama deðerleri grup M, grup Q, grup MQ’da grup K’ya göre düþük olmasýna raðmen arada anlamlý fark yoktu (p>0,05). SONUÇ: Sonuç olarak metilprednizolon, koenzim Q10 ve bu iki ajanýn birlikte kullanýmýnýn ödemi azaltmada faydalý olduðu, koenzim Q10’un deneysel spinal kord hasarýnda, ikincil hasarýn önlenmesinde faydalý olabileceði sonucuna varýldý.

Anahtar Kelimeler: Koenzim Q10, metilprednizolon; sýçanlar, Sprague-Dawley cinsi; spinal kord hasarý.

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Efficency of Coenzym Q10 at Experimental Spinal Cord Injury

Alaeddin Kerimoðlu¹, Özgür Paþaoðlu², Güngör Kanbak³, Volkan Hancý⁴, Filiz Özdemir³, Metin Ant Atasoy^5
1Sarýkamýþ Military Hospital, Department Of Neurosurgery, Kars, Turkey
²Osmangazi University Medical School, Department Of Pathology, Eskiþehir, Turkey
³Osmangazi University Medical School, Department Of Biochemistry, Eskiþehir, Turkey
⁴Sarýkamýþ Military Hospital, Department Of Anesthesiology And Reanimation, Kars, Turkey
⁵Osmangazi University Medical School, Department Of Neurosurgery, Eskiþehir, Turkey

In this study, we aimed to compare the efficacy of methylprednisolone, coenzyme Q10 and combined methylprednisolone and coenzyme Q10 treatments on experimental spinal cord injury. METHODS: Thirty-two male Sprague-Dawley rats (200-250 g) were divided into four groups. Spinal cord injury (SCI) was performed by placement of an aneurysm clip, extradurally at the level of T4-5. After the trauma, group K (control group) received soybean oil, group M (methylprednisolone group) received 30 mg.kg-1 methylprednisolone and 5.4 mg.kg.hour-1 maintenance dose of methylprednisolone, group Q (coenzyme Q10 group) received 10 mg.kg-1 coenzyme Q10, group MQ (methylprednisolone and coenzyme Q10 group) received 30 mg.kg-1 methylprednisolone and 5.4 mg.kg.hour-1 maintenance dose of methylprednisolone and 10 mg.kg-1 coenzyme Q10 intraperitoneally. Twenty-four hours after the trauma spinal cord samples of the rats were obtained and tissue samples had been harvested for both biochemical and histopathological evaluation. RESULTS: In histopathological examination, the edema pattern was significantly more severe in group K than the group M, group Q and group MQ (p<0.001). There was no statistically significant difference between group M, group Q and group MQ regarding edema and bleeding (p>0.05). Mean superoxide dismutase (SOD) scores were significantly low while comparing the group K with all remaining groups and the group MQ comparing with the group M (p<0.05). Mean malondialdehyde (MDA) scores were low in the group M, Q and MQ in comparison with the group K, but there was no statistically significant difference between all groups (p>0.05).
CONCLUSION: Methylprednisolone, coenzyme Q10 and combined methylprednisolone and coenzyme Q10 treatments were found to be effective as they decrease the edema and coenzyme Q10 could be effective for prevention of secondary injury at experimental SCI.

Keywords: Coenzyme Q10, methylprednisolone; rats, Sprague-Dawley; spinal cord injury.

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