Ulus Travma Acil Cerrahi Derg. 2020; 26(3): 373-383 | DOI: 10.14744/tjtes.2019.93428

Deneysel alkali koroziv ösefageal ve mide yanýklarýnda Hyperium perforatum’un (Sarý Kantaron) iyileþtirici etkisi

Erkan Güvenç¹, Selahattin Kýyan², Yiðit Uyanýkgil³, Emel Öykü Çetin⁴, Fatih Karabey⁵, Türker Çavuþoðlu³, Burak Gökçe^6
1Buca Seyfidemirsoy Devlet Hastanesi, Acil Servis Kliniði, Ýzmir
²Ege Üniversitesi Týp Fakültesi Hastanesi, Acil Týp Anabilim Dalý, Ýzmir
³Ege Üniversitesi Týp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalý, Ýzmir
⁴Ege Üniversitesi Eczacýlýk Fakültesi, Farmasötik Teknoloji Anabilim Dalý, Ýzmir
⁵Ege Üniversitesi Fen Bilimleri Enstitüsü, Biyoteknoloji Anabilim Dalý, Ýzmir
⁶Ege Üniversitesi Fen Fakültesi, Biyoloji Anabilim Dalý, Ýzmir

AMAÇ: Koroziv yanýklarda en sýk etiyolojik neden alkali maddelerdir. Hypericum Perforatum’un deneysel özofagus ve mide alkali koroziv yanýk modelinde etkili olup olmadýðý araþtýrýldý.
GEREÇ VE YÖNTEM: Araþtýrmada 42 adet, 200–300 gram aðýrlýðýnda, Wistar Albino sýçanlar seçildi ve 6 grup oluþturuldu; Grup 1: Kontrol, Grup 2: Yanýk+SF (YSF), Grup 3: Yanýk+Kantaron (YK), Grup 4: Yanýk+Plasebo (YP), Grup 5: Kantaron (K), Grup 6: Plasebo (P). Tedavi sonrasýnda 15. gün diseksiyon uygulanarak alýnan özofagus, mide ve karaciðer doku örneklerinden, histopatolojik ve biyokimyasal belirteçlere (SOD, GPX, MDA, CAT) bakýldý. Uygulanan ilaç formulizasyonunun fibroblastlar üzerine sitotoksitesi invitro koþullarda eriþkin insan dermal fibroblast hücre hattýnda deðerlendirildi (HDFa, Gibco invitro hücre kültürü, C-013-5C).
BULGULAR: Deneklerin aðýrlýk deðerlerinin karþýlaþtýrmasýnda Grup 1, 3, 4 ve 6 da aðýrlýk artýþ, Grup 2’de aðýrlýk kaybý saptandý, Grup 5’te ise anlamlý bir fark saptanmadý. YK grubunda özefagusta submukozal kollojen birikimi, muskularis mukoza hasarý, tunika muskularis hasarý ve kollojen akümülasyonu kontrol grubu ile benzerdi, fakat YSF ve plasebodan daha azdý. Midede mukozal hasar, gastrik bez dilatasyonu, submukozal PMNL infiltrasyonu YK grubunda kontrol grubu ile benzer ve YSF grubundan daha az idi. Kantaronun letal kosantrasyonu 2.58 gr/mL idi.
TARTIÞMA: Kantoron özefagus ve midenin orta derecede alkali koroziv yanýklarýnda subakut periyotta korumada etkilidir. Kantaronun gastrointestinal sistemin koroziv yanýklarýnda kullanýlabileceði akýlda tutulmalýdýr.

Anahtar Kelimeler: Alkali, Hypericum Perforatum; koroziv; Saint John’s Wort; yanýk.

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The healing effects of Hyperium perforatum (St. John’s Wort) on experimental alkaline corrosive eosephageal and stomach burns

Erkan Güvenç¹, Selahattin Kýyan², Yiðit Uyanýkgil³, Emel Öykü Çetin⁴, Fatih Karabey⁵, Türker Çavuþoðlu³, Burak Gökçe^6
1Department of Emergency Medicine, Buca Seyfi Demirsoy State Hospital, Ýzmir-Turkey
²Department of Emergency Medicine, Ege University Faculty of Medicine Hospital, Ýzmir-Turkey
³Department of Histology and Embryology, Ege University Faculty of Medicine, Ýzmir-Turkey
⁴Department of Pharmaceutical Technology, Ege University Faculty of Pharmacy, Ýzmir-Turkey
⁵Department of Biotechnology, Ege University Graduate School of Natural and Applied Sciences, Ýzmir-Turkey
⁶Department of Zoology, Ege University Faculty of Science, Ýzmir-Turkey

BACKGROUND: The most frequent etiologic cause is alkaline substances. We investigated the protective effects of the plant St. John ‘s Wort (Hypericum perforatum).
METHODS: We included 42 Wistar albino rats weighing between 200–300 grams and divided into six groups as Group 1: Control, Group 2: Burn+Saline (BS), Group 3: Burn+St. John’s Wort (BSJW), Group 4: Burn+Plasebo (BP), Group 5: St. John’s Wort (SJW), Group 6: Placebo (P). After 15 days of treatment, esophagus, stomach and liver tissue samples were derived by dissection for histopathologic and biochemical markers. The cytotoxic effects of formulation on fibroblasts is evaluated in vitro on human dermoblast fibroblast line (HDFa, Gibco Invitrogen cell culture, C-013-5C).
RESULTS: The weight of the rats increased in Group 1, 3, 4, 6, decreased in Group 2 and did not change in Group 5. In the BSJW group, submucosal collagen accumulation, muscularis mucosa damage, tunica muscularis damage and collagen accumulation in esophagus were similar to the control group but lesser than BS and placebo group. In the stomach, mucosal damage, gastric gland dilatation, submucosal polymorphonuclear infiltration were similar to the control group and lesser than the BS group. The lethal concentration of SJW was 2.58 gr/mL.
CONCLUSION: SJW substrate is effective in protecting the esophagus and stomach in mild to moderate alcali corrosive burns in the subacute period. We should keep in mind the protective effects of STW substrate in alkaline corrosive burns of the gastrointestinal system.

Keywords: Alkaline, burn; corrosive; Hypericum Perforatum; Saint John ‘s Wort.

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