Ulus Travma Acil Cerrahi Derg. 2024; 30(9): 626-634 | DOI: 10.14744/tjtes.2024.77415

Evaluation of the effects of ischemia-reperfusion injury in rat isogenic and allogeneic muscle and skin transplant models

Fatih Ceran¹, Salih Onur Basat², Ýdris Ersin², Deniz Filinte³, Özgür Pilancý², Mehmet Bozkurt^2
1Department of Plastic, Reconstructive and Aesthetic Surgery, Biruni University Medical Faculty, Ýstanbul-Türkiye
²Department of Plastic, Reconstructive and Aesthetic Surgery, Ministry of Health Bagcilar Training & Research Hospital, Ýstanbul-Türkiye
³Department of Pathology Marmara University Medical Faculty, Ýstanbul-Türkiye

BACKGROUND: Ischemia-reperfusion injury (IRI) is a phenomenon that affects transplant survival. The aim of our study was to examine the effects of IRI in isogenic and allogeneic muscle and skin transplantation models exposed to prolonged warm ischemia.
METHODS: Forty-eight Lewis rats and 16 Brown-Norway rats were used to create four groups: Isogenic Inguinal Flap Transplantation (IST), Isogenic Gastrocnemius Muscle Flap Transplantation (IMT), Allogeneic Inguinal Flap Transplantation (AST), and Allogeneic Gastrocnemius Muscle Flap Transplantation (AMT). Malonyldialdehyde (MDA) and superoxide dismutase (SOD) levels were measured on postoperative days 1, 7, 21, 35, 63, 100, and 120 in all groups. Donor-specific chimerism (DSC) in peripheral blood was evaluated in the allogeneic groups on postoperative days 7, 21, 35, 63, 100, and 120. The microRNA-21 and microRNA-205 levels were evaluated on postoperative days 1, 7, and 120 in all groups. At the end of the study, a histopathological examination was performed.
RESULTS: A statistically significant difference was found between the groups in terms of MDA and SOD levels. DSC was detected in the AMT group. A significant increase in microRNA-205 was observed, especially in the AMT group. There was no significant difference in the number of functional muscle units between the muscle transplantation groups.
CONCLUSION: The presence of DSC in the AMT group and the lack of a significant difference in the number of functional muscle units in the IMT and AMT groups are noteworthy findings.

Keywords: Allogeneic, flap; ischemia-reperfusion injury; isogenic; muscle; skin; transplantation.

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Sýçan izojenik ve allojenik kas ve deri transplantasyon modellerinde iskemi-reperfüzyon hasarýnýn etkilerinin deðerlendirilmesi

Fatih Ceran¹, Salih Onur Basat², Ýdris Ersin², Deniz Filinte³, Özgür Pilancý², Mehmet Bozkurt^2
1Biruni Üniversitesi Týp Fakültesi, Plastik, Rekonstrüktif ve Estetik Cerrahi Anabilim Dalý, Ýstanbul, Türkiye
²Saðlýk Bakanlýðý Baðcýlar Eðitim ve Araþtýrma Hastanesi, Plastik, Rekonstrüktif ve Estetik Cerrahi Anabilim Dalý, Ýstanbul, Türkiye
³Marmara Üniversitesi Týp Fakültesi, Patoloji Anabilim Dalý, Ýstanbul, Türkiye

AMAÇ: Ýskemi-reperfüzyon hasarý, transplantasyon baþarýsýný etkileyen bir durumdur. Çalýþmamýzýn amacý, uzun süreli sýcak iskemiye maruz kalan izojenik ve allojenik kas ve deri transplantasyon modellerinde iskemi-reperfüzyon hasarýnýn etkilerini incelemektir.
GEREÇ VE YÖNTEM: 48 Lewis ve 16 Brown-Norway sýçan dört grup oluþturmak üzere kullanýldý: IST: Ýzojenik inguinal flep transplantasyonu, IMT: Ýzojenik gastroknemius kas flebi transplantasyonu, AST: Allojenik inguinal flep transplantasyonu ve AMT: Allojenik gastroknemius kas flebi transplantasyonu. Tüm gruplarda postoperatif 1., 7., 21., 35., 63., 100. ve 120. günlerde malondialdehit (MDA) ve süperoksit dismutaz (SOD) deðerleri ölçüldü. Allojenik gruplarda postoperatif 7., 21., 35., 63., 100. ve 120. günlerde periferik kanda donör spesifik kimerizm (DSC) deðerlendirildi. Tüm gruplarda postoperatif 1., 7. ve 120. günlerde miRNA-21 ve miRNA-205 düzeyleri deðerlendirildi. Çalýþmanýn sonunda histopatolojik çalýþma yapýldý. BULGULAR: Gruplar arasýnda MDA ve SOD deðerleri açýsýndan istatistiksel olarak anlamlý fark bulundu. AMT grubunda DSC tespit edildi. Özellikle AMT grubunda miRNA-205'te anlamlý artýþ gözlendi. Kas transplantasyon gruplarý arasýnda fonksiyonel kas ünitesi sayýsý açýsýndan anlamlý bir fark yoktu.
SONUÇ: AMT grubunda DSC varlýðý ve IMT ve AMT gruplarýnda fonksiyonel kas ünitesi sayýsý arasýnda anlamlý bir fark olmamasý dikkat çekici bulgulardýr.

Anahtar Kelimeler: Allojenik, deri; flep; iskemi-reperfüzyon hasarý; izojenik; kas; transplantasyon.

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