Ulus Travma Acil Cerrahi Derg. 2014; 20(3): 151-160 | DOI: 10.5505/tjtes.2014.96493

Captopril protects against burn-induced cardiopulmonary injury in rats

Esra Saglam¹, Ahmet Ozer Sehirli², Emine Nur Ozdamar³, Gazi Contuk⁴, Sule Cetinel⁴, Derya Ozsavcý⁵, Selami Suleymanoglu⁶, Goksel Sener^2
1Uskudar University, Istanbul Neuropsychiatry Hospital, Laboratory Of Clinical Pharmacogenetics, Istanbul, Turkey
²Marmara University, School Of Pharmacy, Department Of Pharmacology, Istanbul, Turkey
³Marmara University, School Of Medicine, Department Of Medical Pharmacology, Istanbul, Turkey
⁴Marmara University, School Of Medicine, Department Of Histology & Embryology, Istanbul, Turkey
⁵Marmara University, School Of Pharmacy, Department Of Biochemistry, Istanbul, Turkey
⁶Gulhane Military Medical Academy, Department Of Cardiology, Istanbul, Turkey

BACKGROUND: This study was designed to determine the possible protective effect of captopril treatment against oxidative damage in heart and lung tissues induced by burn injury.
METHODS: Under ether anesthesia, the shaved dorsum of Wistar albino rats was exposed to 90°C water bath for 10 seconds. Captopril was administered intraperitoneally (10 mg/kg) after the burn injury and repeated twice daily. In the sham group, the dorsum was dipped in a 25°C water bath for 10 seconds. At the end of the 24 hours, echocardiographic recordings were performed, then animals were decapitated and heart and lung tissue samples were taken for the determination of tumor necrosis factor-α (TNF-α) as a pro-inflammatory cytokine, malondialdehyde and glutathione levels and myeloperoxidase, caspase-3, and Na+,K+-ATPase activity in addition to the histological analysis.
RESULTS: Burn injury caused significant alterations in left ventricular function. In heart and lung tissues, TNF-α and malondialdehyde levels and myeloperoxidase and caspase-3 activities were found to be increased, while glutathione levels and Na+, K+-ATPase activity were decreased due to burn injury. Captopril treatment significantly elevated the reduced glutathione level and Na+, K+-ATPase activity, and decreased cytokine and malondialdehyde levels and myeloperoxidase and caspase-3 activities.
CONCLUSION: Captopril prevents burn-induced damage in heart and lung tissues and protects against oxidative organ damage.

Keywords: Captopril, cytokine; lipid peroxidation; myeloperoxidase; thermal trauma.

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Sýçanlarda yanýkla uyarýlan kardiyopulmoner hasara karþý kaptoprilin koruyucu etkisi

Esra Saglam¹, Ahmet Ozer Sehirli², Emine Nur Ozdamar³, Gazi Contuk⁴, Sule Cetinel⁴, Derya Ozsavcý⁵, Selami Suleymanoglu⁶, Goksel Sener^2
1Üsküdar Üniversitesi, Ýstanbul Nöropsikiyatri Hastanesi, Klinik Farmakogenetik Laboratuarý, Ýstanbul, Türkiye
²Marmara Üniversitesi Eczacýlýk Fakültesi, Farmakoloji Anabilim Dalý, Ýstanbul, Türkiye
³Marmara Üniversitesi Týp Fakültesi, Týbbi Farmakoloji Anabilim Dalý, Ýstanbul, Turkiye
⁴Marmara Üniversitesi Týp Fakültesi, Histoloji & Embriyoloji Anabilim Dalý, Ýstanbul, Turkiye
⁵Marmara Üniversitesi Eczacýlýk Fakültesi, Biokimya Anabilim Dalý, Ýstanbul, Turkiye
⁶Gülhane Askeri Týp Akademisi, Kardiyoloji Anabilim Dalý, Ýstanbul, Turkiye

AMAÇ: Bu çalýþma, kalp ve akciðer dokularýnda yanýkla uyarýlan oksidatif hasara karþý kaptopril tedavisinin olasý koruyucu etkisini saptamak için tasarlandý.
GEREÇ VE YÖNTEM: Eter anestezisi altýnda sýrt derileri traþ edilen Wistar albino türü sýçanlar 10 saniye süreyle 90°C suya tutuldu. Yanýk hasarýndan sonra ve 12 saat sonra olmak üzere 10 mg/kg intraperitoneal kaptopril uygulandý. Kontrol grubunda ise sýçanlarýn sýrtý 10 saniye süreyle 25°C suya tutuldu. Yanýk hasarýndan 24 saat sonra ekokardiyografik kayýtlarý alýnan sýçanlar dekapite edildi ve kalp ve akciðer doku örnekleri çýkartýlarak tümör nekroz faktör-α (TNF-α), malondialdehit ve glutatyon düzeyleri, miyeloperoksidaz, kaspaz-3 ve Na+, K+-ATPaz aktiviteleri tayini ile histolojik analizler yapýldý.
BULGULAR: Yanýk sol ventrikül fonksiyonlarýnda önemli deðiþikliklere neden oldu. Kalp ve akciðer dokularýnda yanýk hasarýna baðlý olarak glutatyon düzeyleri ve Na+, K+-ATPaz aktivitelerinin azaldýðý ve TNF-α ve malondialdehit düzeyleri ile miyeloperoksidaz ve kaspaz-3 aktivitelerinin arttýðý bulundu. Kaptopril tedavisinin, azalmýþ glutatyon düzeyi ve Na+, K+-ATPaz aktivitesini anlamlý düzeyde yükselttiði ve sitokin ve malondialdehit düzeyleri ve miyeloperoksidaz ve kaspaz-3 aktivitelerini ise anlamlý düzeyde azalttýðý bulundu.
TARTIÞMA: Kaptopril kalp ve akciðer dokularýnda yanýkla uyarýlan hasarý önler ve oksidatif organ hasarýna karþý korur.

Anahtar Kelimeler: Kaptopril, lipid peroksidasyonu; miyeloperoksidaz; sitokin; yanýk.

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