Objective: To date, no study has evaluated the effects of calcium dobesilate on regenerative capacity after partial hepatectomy. Within the scope of this research, we aimed to elucidate the effects of calcium dobesilate (CD) on liver regeneration capacity and antioxidant pathways after partial hepatectomy.

Method: Thirty-six Spraque Dawley male rats weighing between 250-350 grams were used in the study. All animals underwent partial hepatectomy. The rats were randomly divided into four groups, each consisting of 9 rats as the control groups (Groups 1 and 2) and study groups (Groups 3 and 4). Regeneration rate, histopathological parameters, an immunohistochemical examination, and the Apoptotic Index (AI) were measured.

Results: The tissue superoxide dismutase (SOD) level was statistically significantly higher in the calcium dobesilate study groups compared to controls (p=0.03). Malondialdehyde (MDA) was statistically significantly higher in the study groups than in the control groups on both the 2nd and 7th days (p=0.001). The regeneration rate (RR) was higher in the study group compared to the control group on the 2nd day, and this difference was statistically significant (p<0.001). RR was significantly higher in the study group on the 2nd day compared to the 7th day group (p<0.001). According to the Suzuki Scoring System, vacuolization and necrosis were not observed in the study groups (p<0.001 vs. p=0.034, respectively). Apoptotic index (AI) was significantly higher in the control groups compared to the study groups (p<0.001), and AI was statistically significantly lower on the 7th day (p=0.006). Ki67 expression was statistically significantly higher in the groups given CD treatment on the 2nd and 7th days. In control groups, it was statistically significantly higher on the 7th day compared to the 2nd day (p=0.006).

Conclusion: This research indicated the effects of calcium dobesilate on improving oxidative damage and liver regeneration in rats with partial hepatectomy. Results of the present study showed that (preoperative-postoperative) CD improves oxidative stress and increases liver regeneration capacity after partial hepatectomy.

Keywords: Calcium dobesilate, liver regeneration, oxidative damage, partial hepatectomy, experimental.

Parsiyel Hepatektomi Yapýlan Ratlarda Kalsiyum Dobesilatýn Karaciðer Rejenerasyonu Üzerine Etkisi

Amaç: Bugüne kadar hiçbir çalýþma, kalsiyum dobesilatýn parsiyel hepatektomi sonrasý rejeneratif kapasite üzerindeki etkilerini deðerlendirmemiþtir. Bu araþtýrma kapsamýnda, kalsiyum dobesilatýn (KD) parsiyel hepatektomi sonrasý karaciðer rejenerasyon kapasitesi ve antioksidan yolaklarý üzerindeki etkilerini açýklamayý amaçladýk.

Yöntem: Çalýþmada 250 ile 350 gram aðýrlýðýnda otuz altý Spraque Dawley erkek sýçan kullanýldý. Tüm hayvanlara parsiyel hepatektomi uygulandý. Sýçanlar rastgele dört gruba ayrýldý, her grup kontrol grubu (Grup 1 ve 2) ve çalýþma grubu (Grup 3 ve 4) olmak üzere 9 sýçandan oluþuyordu. Rejenerasyon oraný, histopatolojik parametreler, immünohistokimyasal inceleme ve Apoptotik Ýndeks (AÝ) ölçüldü.

Bulgular: Doku süperoksit dismutaz (SOD) seviyesi, çalýþma gruplarýnda kontrol gruplarýna kýyasla istatistiksel olarak anlamlý derecede yüksekti (p=0.03). Çalýþma gruplarýnda malondialdehit (MDA) hem 2. hem de 7. günde kontrol gruplarýna kýyasla istatistiksel olarak anlamlý derecede yüksekti (p=0.001). 2. günde rejenerasyon oraný (RO), çalýþma grubunda kontrol grubuna kýyasla daha yüksekti ve bu fark istatistiksel olarak anlamlýydý (p<0.001). 2. günde RO, KD grubunda 7. gün grubuna kýyasla anlamlý derecede yüksekti (p<0.001). Suzuki Skorlama Sistemine göre çalýþma gruplarýnda vakuolizasyon ve nekroz gözlenmedi (sýrasýyla p<0.001'e karþý p=0.034). Apoptotik indeks (AÝ), kontrol gruplarýnda çalýþma gruplarýna kýyasla anlamlý derecede yüksekti (p<0.001) ve AI, 7. günde istatistiksel olarak anlamlý derecede düþüktü (p=0.006). Ki67 ekspresyonu çalýþma gruplarýnda 2. ve 7. günde istatistiksel olarak anlamlý derecede yüksekti. Kontrol gruplarýnda 7. günde 2. güne göre istatistiksel olarak anlamlý derecede daha yüksekti (p=0.006).

Sonuç: Bu araþtýrma, kalsiyum dobesilatýn parsiyel hepatektomi uygulanan sýçanlarda oksidatif hasarý iyileþtirme ve karaciðer rejenerasyonunu artýrma üzerindeki etkilerini ortaya koymuþtur. Mevcut çalýþmanýn sonuçlarý, (ameliyat öncesi ve sonrasý) KD'nin parsiyel hepatektomi sonrasý oksidatif stresi iyileþtirdiðini ve karaciðer rejenerasyon kapasitesini artýrdýðýný göstermiþtir.

Anahtar Kelimeler: Kalsiyum dobesilat, karaciðer rejenerasyonu, oksidatif hasar, parsiyel hepatektomi, deneysel.